Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-MAGEC2 antibody [EPR19064] - BSA and Azide free
See all MAGEC2 primary antibodies
Description

Rabbit monoclonal [EPR19064] to MAGEC2 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, IP, Flow Cyt, ICC/IF, WBmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A-375 whole cell lysate; Full length recombinant protein MAGEE1. IHC-P: Human testis, gastric cancer and melanoma tissues. ICC/IF: A-375 cells. Flow Cyt: A-375 cells. IP: A-375 whole cell lysate.
General notes
Ab223794 is the carrier-free version of ab209667. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab223794 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19064
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] - BSA and Azide free (ab223794)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human germ cells is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209667).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] - BSA and Azide free (ab223794)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human stomach. Counter stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209667).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] - BSA and Azide free (ab223794)

Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on melanoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209667).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-MAGEC2 antibody [EPR19064] - BSA and Azide free (ab223794)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-375 (Human malignant melanoma cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling MAGEC2 with ab209667 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear and weakly cytoplasmic staining on A-375 cell line

Negative expression in HeLa cells.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows:
-ve control 1: ab209667 at 1/500 dilution followed by ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor^®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209667).
Flow Cytometry - Anti-MAGEC2 antibody [EPR19064] - BSA and Azide free (ab223794)

Flow cytometric analysis of 4% paraformaldehyde-fixed A-375 (Human malignant melanoma cell line) cells labeling MAGEC2 with ab209667 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/500 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209667).
Immunoprecipitation - Anti-MAGEC2 antibody [EPR19064] - BSA and Azide free (ab223794)

MAGEC2 was immunoprecipitated from 1mg of A-375 (Human malignant melanoma cell line) whole cell lysate with ab209667 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209667 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: A-375 whole cell lysate 10µg (Input).

Lane 2: ab209667 IP in A-375 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209667 in A-375 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209667).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] - BSA and Azide free (ab223794)

This IHC data was generated using the same anti-MAGEC2 antibody clone [EPR19064] in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (cat# ab209667).

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human gastric cancer is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-MAGEC2 antibody [EPR19064] - BSA and Azide free (ab223794)