Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAGEC2 antibody [EPR19064] (ab209667)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19064] to MAGEC2
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Human, Recombinant fragment

Overview

  • Product name

    Anti-MAGEC2 antibody [EPR19064]
    See all MAGEC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR19064] to MAGEC2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A-375 whole cell lysate; Full length recombinant protein MAGEE1. IHC-P: Human testis, gastric cancer and melanoma tissues. ICC/IF: A-375 cells. Flow Cyt: A-375 cells. IP: A-375 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19064

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (ab209667)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (ab209667)

    Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human gastric cancer is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAGEC2 antibody [EPR19064] (ab209667)
    Immunocytochemistry/ Immunofluorescence - Anti-MAGEC2 antibody [EPR19064] (ab209667)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-375 (Human malignant melanoma cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling MAGEC2 with ab209667 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear and weakly cytoplasmic staining on A-375 cell line

    Negative expression in HeLa cells.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).


    The negative controls are as follows:
    -ve control 1: ab209667 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Western blot - Anti-MAGEC2 antibody [EPR19064] (ab209667)
    Western blot - Anti-MAGEC2 antibody [EPR19064] (ab209667)
    All lanes : Anti-MAGEC2 antibody [EPR19064] (ab209667) at 1/10000 dilution

    Lane 1 : A-375 (Human malignant melanoma cell line) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 4 : Mouse testis lysate
    Lane 5 : Human fetal kidney lysate
    Lane 6 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 41 kDa
    Observed band size: 51 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The band is consistent with what is observed in the literature (PMID: 16594646).

  • Western blot - Anti-MAGEC2 antibody [EPR19064] (ab209667)
    Western blot - Anti-MAGEC2 antibody [EPR19064] (ab209667)
    Anti-MAGEC2 antibody [EPR19064] (ab209667) at 1/5000 dilution + Full length recombinant protein MAGEC2 at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 41 kDa
    Observed band size: 51 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (ab209667)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (ab209667)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human germ cells is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (ab209667)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (ab209667)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human stomach. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (ab209667)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (ab209667)

    Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on melanoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-MAGEC2 antibody [EPR19064] (ab209667)
    Flow Cytometry - Anti-MAGEC2 antibody [EPR19064] (ab209667)

    Flow cytometric analysis of 4% paraformaldehyde-fixed A-375 (Human malignant melanoma cell line) cells labeling MAGEC2 with ab209667 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-MAGEC2 antibody [EPR19064] (ab209667)
    Immunoprecipitation - Anti-MAGEC2 antibody [EPR19064] (ab209667)

    MAGEC2 was immunoprecipitated from 1mg of A-375 (Human malignant melanoma cell line) whole cell lysate with ab209667 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209667 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: A-375 whole cell lysate 10µg (Input).

    Lane 2: ab209667 IP in A-375 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209667 in A-375 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-MAGEC2 antibody [EPR19064] (ab209667)
    Anti-MAGEC2 antibody [EPR19064] (ab209667)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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