Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-BRCA1 antibody [EPR19433] (ab213929)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19433] to BRCA1
Suitable for: IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-BRCA1 antibody [EPR19433]
See all BRCA1 primary antibodies
Description

Rabbit monoclonal [EPR19433] to BRCA1
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

IHC-P
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human breast, breast cancer and ovary cancer tissues. ICC/IF: MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19433
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [EPR19433] (ab213929)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling BRCA1 with ab213929 at 1/400 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Strong nuclear staining on epithelium cells of human breast is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [EPR19433] (ab213929)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling BRCA1 with ab213929 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on MCF7 cell line. The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Negative control: No staining on SUM149PT cell line, which carried the 2288delT mutation of BRCA1 [PMID: 16397213].
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [EPR19433] (ab213929)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling BRCA1 with ab213929 at 1/400 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Lower nuclear staining intensity in tumor cells (left) as compared to its adjacent non-tumor tissue (right).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [EPR19433] (ab213929)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling BRCA1 with ab213929 at 1/400 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Strong nuclear staining on a region of tumor cells from human breast cancer tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [EPR19433] (ab213929)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling BRCA1 with ab213929 at 1/400 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Absent staining on this case of human breast cancer is observed.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [EPR19433] (ab213929)

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue labeling BRCA1 with ab213929 at 1/400 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Strong nuclear staining on a region of tumor cells from human ovary cancer tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-BRCA1 antibody [EPR19433] (ab213929)

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