Anti-BRCA1 antibody (ab9141)
Key features and details
- Rabbit polyclonal to BRCA1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BRCA1 antibody
See all BRCA1 primary antibodies -
Description
Rabbit polyclonal to BRCA1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide (Human) - which represents aportion within the last 100 amino acids of the human Breast Cancer Gene 1 (GenBank PID 627392), conjugated to KLH.
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Positive control
- WB: HEK-293T cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using thepeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRCA1 antibody (ab9141)Anti-BRCA1 antibody (ab9141) at 0.04 µg/ml + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer) at 50 µg
Predicted band size: 220 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-BRCA1 antibody (ab9141)Detection of BRCA1 by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer.
Lane 1: Anti-BRCA1 antibody (ab9141) used for IP at 6 µg per reaction.
Lane 2: IgG Control
For blotting immunoprecipitated BRCA1, ab9141 was used at 0.04 µg/mL.
Detection: Chemiluminescence with an exposure time of 75 seconds.
