Anti-Rad6 antibody (ab31917)
Key features and details
- Rabbit polyclonal to Rad6
- Suitable for: IHC-M, IHC-P, WB
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-Rad6 antibody
See all Rad6 primary antibodies -
Description
Rabbit polyclonal to Rad6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-M, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Zebrafish
Predicted to work with: Rabbit, Chicken, Cow, Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster
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Immunogen
Synthetic peptide corresponding to Human Rad6 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab28495)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab31917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-M Use at an assay dependent concentration. IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). Target
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Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. - Information by UniProt
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Database links
- Entrez Gene: 395672 Chicken
- Entrez Gene: 282107 Cow
- Entrez Gene: 7319 Human
- Entrez Gene: 22209 Mouse
- Entrez Gene: 298317 Rat
- Entrez Gene: 398788 Xenopus laevis
- Entrez Gene: 797853 Zebrafish
- Omim: 312180 Human
see all -
Alternative names
- BHR6A antibody
- hHR6A antibody
- HR6A antibody
see all
Images
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Western blot - Anti-Rad6 antibody (ab31917)All lanes : Anti-Rad6 antibody (ab31917) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 17 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad6 antibody (ab31917)IHC image of ab31917 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31917, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Rad6 antibody (ab31917)All lanes : Anti-Rad6 antibody (ab31917) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate
Lane 3 : Zebrafish heart homogenate
Lane 4 : Zebrafish liver homogenate
Lane 5 : Zebrafish skeletal muscle homogenate
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 5 minutes -
Western blot - Anti-Rad6 antibody (ab31917)All lanes : Anti-Rad6 antibody (ab31917) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
References (6)
ab31917 has been referenced in 6 publications.
- Yamada T et al. Systematic Analysis of Targets of Pumilio-Mediated mRNA Decay Reveals that PUM1 Repression by DNA Damage Activates Translesion Synthesis. Cell Rep 31:107542 (2020). WB . PubMed: 32375027
- Shen JD et al. High expression of ubiquitin-conjugating enzyme E2A predicts poor prognosis in hepatocellular carcinoma. Oncol Lett 15:7362-7368 (2018). PubMed: 29725449
- Rosner K et al. Melanoma Development and Progression Are Associated with Rad6 Upregulation and ß -Catenin Relocation to the Cell Membrane. J Skin Cancer 2014:439205 (2014). IHC-P ; Human . PubMed: 24891954
- Read ML et al. The PTTG1-binding factor (PBF/PTTG1IP) regulates p53 activity in thyroid cells. Endocrinology 155:1222-34 (2014). PubMed: 24506068
- Masuda Y et al. Asymmetric nature of two subunits of RAD18, a RING-type ubiquitin ligase E3, in the human RAD6A-RAD18 ternary complex. Nucleic Acids Res 40:1065-76 (2012). WB . PubMed: 21967848
- Masuda Y et al. En bloc transfer of polyubiquitin chains to PCNA in vitro is mediated by two different human E2-E3 pairs. Nucleic Acids Res 40:10394-407 (2012). WB ; Human . PubMed: 22904075
Images
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Western blot - Anti-Rad6 antibody (ab31917)All lanes : Anti-Rad6 antibody (ab31917) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 17 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad6 antibody (ab31917)IHC image of ab31917 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31917, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Rad6 antibody (ab31917)All lanes : Anti-Rad6 antibody (ab31917) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate
Lane 3 : Zebrafish heart homogenate
Lane 4 : Zebrafish liver homogenate
Lane 5 : Zebrafish skeletal muscle homogenate
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 5 minutes
-
Western blot - Anti-Rad6 antibody (ab31917)All lanes : Anti-Rad6 antibody (ab31917) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 3 minutes
