Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23077-14] to UBE3A - BSA and Azide free
  • Suitable for: WB, ICC/IF, ELISA, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free
    See all UBE3A primary antibodies

  • Description

    Rabbit monoclonal [EPR23077-14] to UBE3A - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, K562, HepG2,A549, HEK-293T, PC-12, PC-12 (treated with 10 uM MG-132 for 4 hours), RAW 264.7 and RAW 264.7 (treated with 10 uM MG-132 for 4 hours) whole cell lysates; Mouse spleen tissue lysate;, Rat brain tissue lysate. ICC/IF: HeLa and RAW 264.7 cells. Flow Cyt: HeLa and RAW 264.7 cells. IP: K562 and RAW 264.7 whole cell lysates.

  • General notes

    ab272173 is the carrier-free version of ab272168. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
    Immunocytochemistry/ Immunofluorescence - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling UBE3A with ab272168 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weak cytoplasmic staining in HeLa cell line ab195889. Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272168).

  • ELISA - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
    ELISA - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
    indirect ELISA using ab272168 at varying antibody concentrations (1000-0 ng/ml) and Human UBE3A antigen at 1000 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as a secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272168).

  • Flow Cytometry - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
    Flow Cytometry - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling UBE3A with ab272168 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272168).

  • Immunoprecipitation - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
    Immunoprecipitation - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

    UBE3A was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab272168 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272168 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug

    Lane 2: ab272168 IP in K-562 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272168 in K-562 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

    A 37 kDa degraded band is observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272168).

  • Immunocytochemistry/ Immunofluorescence - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
    Immunocytochemistry/ Immunofluorescence - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling UBE3A with ab272168 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weak cytoplasmic staining in RAW 264.7 cell line. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272168).

  • Flow Cytometry - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
    Flow Cytometry - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cells labelling UBE3A with ab272168 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272168).

  • Immunoprecipitation - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
    Immunoprecipitation - Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

    UBE3A was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) (treated with 10 μM MG-132 for 4 hours) whole cell lysate with ab272168 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272168 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 10 μM MG-132 for 4 hours, whole cell lysate 10 ug

    Lane 2: ab272168 IP in RAW 264.7 treated with 10 μM MG-132 for 4 hours, whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272168 in RAW 264.7 treated with 10 μM MG-132 for 4 hours, whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    A 37 kDa degraded band is observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272168).

  • Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)
    Anti-UBE3A antibody [EPR23077-14] - BSA and Azide free (ab272173)

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