Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UBE3A antibody [EPR7330] (ab126765)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7330] to UBE3A
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UBE3A antibody [EPR7330]
    See all UBE3A primary antibodies

  • Description

    Rabbit monoclonal [EPR7330] to UBE3A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide, corresponding to residues in Human UBE3A (Q05086).

  • Positive control

    • Jurkat, HeLa, and K562 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-UBE3A antibody [EPR7330] (ab126765)
    Western blot - Anti-UBE3A antibody [EPR7330] (ab126765)
    All lanes : Anti-UBE3A antibody [EPR7330] (ab126765) at 1/10000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Rat spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size: 100 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-UBE3A antibody [EPR7330] (ab126765)
    Western blot - Anti-UBE3A antibody [EPR7330] (ab126765)
    All lanes : Anti-UBE3A antibody [EPR7330] (ab126765) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size: 100 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-UBE3A antibody [EPR7330] (ab126765)
    Western blot - Anti-UBE3A antibody [EPR7330] (ab126765)
    All lanes : Anti-UBE3A antibody [EPR7330] (ab126765) at 1/1000 dilution (unpuified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 100 kDa

  • OI-RD Scanning - Anti-UBE3A antibody [EPR7330] (ab126765)
    OI-RD Scanning - Anti-UBE3A antibody [EPR7330] (ab126765)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-UBE3A antibody [EPR7330] (ab126765)
    Anti-UBE3A antibody [EPR7330] (ab126765)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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