Antibodies, Proteins, Kits and Reagents for Life Science

Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18657] to MYSM1 - BSA and Azide free
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-MYSM1 antibody [EPR18657] - BSA and Azide free
See all MYSM1 primary antibodies
Description

Rabbit monoclonal [EPR18657] to MYSM1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251165 is the carrier-free version of ab193081. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251165 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR18657
Isotype

IgG
Research areas

Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165)

All lanes : Anti-MYSM1 antibody [EPR18657] (ab193081) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 5 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 66, 95 kDa
Observed band size: 66,95 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

This data was developed using ab193081, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Two bands detected represent two isoforms.
Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165)

Anti-MYSM1 antibody [EPR18657] (ab193081) at 1/1000 dilution + mESC (Mouse embryonic stem cell line) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 66, 95 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?

Exposure time: 8 seconds

This data was developed using ab193081, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165)

All lanes : Anti-MYSM1 antibody [EPR18657] (ab193081) at 1/1000 dilution

Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 66, 95 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

This data was developed using ab193081, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165)

This data was developed using ab193081, the same antibody clone in a different buffer formulation.

MYSM1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab193081 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab193081 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: HeLa whole cell lysate 10µg (Input).

Lane 2: ab193081 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab193081 in HeLa whole cell lysate.

Predicted band size: 66kDa, 95kDa.

Observed band size: 66kDa, 95kDa.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

Two bands detected represent two isoforms.
Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165)

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