Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp27 antibody [CPTC-HSPB1-2] - BSA and Azide free (ab268194)

Key features and details

  • Mouse monoclonal [CPTC-HSPB1-2] to Hsp27 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, IHC-P, Protein Array
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Hsp27 antibody [CPTC-HSPB1-2] - BSA and Azide free
    See all Hsp27 primary antibodies

  • Description

    Mouse monoclonal [CPTC-HSPB1-2] to Hsp27 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-P, Protein Arraymore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein within Human Hsp27 aa 1-205. The exact sequence is proprietary.
    Database link: P04792

  • Positive control

    • ICC/IF: MCF-7 cells. IHC-P: Human prostate tissue. Flow Cyt: MCF-7 cells.

  • General notes

    ab268194 is the PBS only version of ab268073.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [CPTC-HSPB1-2] - BSA and Azide free (ab268194)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [CPTC-HSPB1-2] - BSA and Azide free (ab268194)

    Immunofluorescence staining of PFA-fixed MCF-7 (Human breast adenocarcinoma cell line) cells labeling Hsp27 with ab268073 at 2 μg/ml followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268073)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [CPTC-HSPB1-2] - BSA and Azide free (ab268194)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [CPTC-HSPB1-2] - BSA and Azide free (ab268194)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for Hsp27 using ab268073 at 2 μg/ml in immunohistochemical analysis

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268073)

  • Flow Cytometry - Anti-Hsp27 antibody [CPTC-HSPB1-2] - BSA and Azide free (ab268194)
    Flow Cytometry - Anti-Hsp27 antibody [CPTC-HSPB1-2] - BSA and Azide free (ab268194)

    Flow Cytometic analysis of PFA-fixed MCF-7 (Human breast adenocarcinoma cell line) cells labeling HSp27 using ab268073 at 2 µg/million cells followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268073).

  • Protein Array - Anti-Hsp27 antibody [CPTC-HSPB1-2] - BSA and Azide free (ab268194)
    Protein Array - Anti-Hsp27 antibody [CPTC-HSPB1-2] - BSA and Azide free (ab268194)

    Protein Array containing more than 19,000 full-length human proteins using ab268073.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268073)

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