Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [RcMab-1] to IDH1 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-IDH1 antibody [RcMab-1] - BSA and Azide free
    See all IDH1 primary antibodies

  • Description

    Rat monoclonal [RcMab-1] to IDH1 - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant fragment within Human IDH1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: O75874

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human prostatic hyperplasia tissue and kidney tissue. WB: Wild type HAP1 whole cell lysate. HeLa, PC-12, HepG2 and Raji whole cell lysate. Human and rat heart tissue lysate. ICC/IF: Wild type HAP1 cells.

  • General notes

    ab256562 is the carrier-free version of ab256557. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256562 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
    Western blot - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
    All lanes : Anti-IDH1 antibody [RcMab-1] (ab256557) at 1.479 µg/ml

    Lane 1 : Wild type HAP1 whole cell lysate
    Lane 2 : IDH1 knockout HAP1 whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 5 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 46 kDa


    Exposure time: 8 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256557).

  • Western blot - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
    Western blot - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
    All lanes : Anti-IDH1 antibody [RcMab-1] (ab256557) at 1.479 µg/ml

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
    Lane 2 : Rat heart tissue lysate
    Lane 3 : Human heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 46 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lanes 2-3: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256557).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)

    Immunohistochemical analysis of paraffin-embedded human prostatic hyperplasia tissue labeling IDH1 with ab256557 at 1/500 dilution (14.79µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on human prostatic hyperplasia. The section was incubated with ab256557 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256557).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling IDH1 with ab256557 at 1/500 dilution (14.79µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on human kidney tissue. The section was incubated with ab256557 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256557).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)

    Immunohistochemical analysis of paraffin-embedded human normal cerebrum tissue labeling IDH1 with ab256557 at 1/500 dilution (14.79µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). The section was incubated with ab256557 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Negative control: No staining on human normal cerebrum.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256557).

  • Immunocytochemistry/ Immunofluorescence - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
    Immunocytochemistry/ Immunofluorescence - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized wild-type HAP1 cells labelling IDH1 with ab256557 at 1/20 dilution (73.95µg/ml), followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

    Confocal image showing positive staining in wild-type HAP1 cells, while no staining in IDH1 knockout HAP1 cells.

    ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only controls.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256557).

  • Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
    Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)

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