Anti-PSRC2 antibody [EPR20913-32] - BSA and Azide free (ab270035)
![Anti-PSRC2 antibody [EPR20913-32] - BSA and Azide free (ab270035)](https://www.abcam.com/ps/products/270/ab270035/Images/ab270035-451681-anti-psrc2-antibody-epr2091332-western-blot-lysate.jpg "Anti-PSRC2 antibody [EPR20913-32] - BSA and Azide free (ab270035)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20913-32] to PSRC2 - BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PSRC2 antibody [EPR20913-32] - BSA and Azide free
See all PSRC2 primary antibodies -
Description
Rabbit monoclonal [EPR20913-32] to PSRC2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lymph node(WT) and testis tissue lysates; Rat thymus tissue lysate; HeLa; 293T whole cell lysates.
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General notes
ab270035 is the carrier-free version of ab236629. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab270035 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20913-32 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PSRC2 antibody [EPR20913-32] - BSA and Azide free (ab270035)All lanes : Anti-PSRC2 antibody [EPR20913-32] (ab236629) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat thymus tissue lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 226 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?This data was developed using ab236629, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 15 seconds
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![Western blot - Anti-PSRC2 antibody [EPR20913-32] - BSA and Azide free (ab270035)](https://www.abcam.com/ps/products/270/ab270035/Images/ab270035-451680-anti-psrc2-antibody-epr2091332-western-blot-tissue.jpg)
Western blot - Anti-PSRC2 antibody [EPR20913-32] - BSA and Azide free (ab270035)All lanes : Anti-PSRC2 antibody [EPR20913-32] (ab236629) at 1/1000 dilution
Lane 1 : Mouse lymph node(WT) tissue lysate
Lane 2 : PSRC2 knockout mouse lymph node tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 226 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?This data was developed using ab236629, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were kindly provided by collaborator.
ab236629 Anti-PSRC2 antibody [EPR20913-32] was shown to react with PSRC2 in mouse lymph node tissue lysate in Western blot. Loss of signal was observed when PSRC2 knockout sample was used. Wild-type and PSRC2 knockout samples were subjected to SDS-PAGE. ab236629 and ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100, 000 dilution for 1 hour at room temperature before imaging. he blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Exposure time: 15 seconds