Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CSC1 antibody [EPR19814] (ab203486)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19814] to CSC1
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CSC1 antibody [EPR19814]
    See all CSC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19814] to CSC1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    This product was produced with the following immunogens:
    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    Recombinant fragment within Mouse CSC1 aa 200-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q8CBX0

    Recombinant fragment within Mouse CSC1 aa 700 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q8CBX0

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Mouse and rat cerebral cortex and cerebellum lysates; CSC1 WT (+/+) mouse brain lysate. ICC/IF: mCSC1 transfected HEK-293T cells. IP: Mouse and rat cerebellum lysates.

  • General notes

     This product was previously labelled as TMEM63C

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19814

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CSC1 antibody [EPR19814] (ab203486)
    Western blot - Anti-CSC1 antibody [EPR19814] (ab203486)
    All lanes : Anti-CSC1 antibody [EPR19814] (ab203486) at 1/1000 dilution

    Lane 1 : Mouse cerebral cortex lysate
    Lane 2 : Mouse cerebellum lysate
    Lane 3 : Rat cerebellum lysate
    Lane 4 : Rat cerebral cortex lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 93 kDa
    Observed band size: 93 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2, 3 and 4: 10 seconds.

    To avoid the formation of CSC1 high molecule weight aggregates the lysates in sample buffer were not boiled prior to WB testing.

  • Western blot - Anti-CSC1 antibody [EPR19814] (ab203486)
    Western blot - Anti-CSC1 antibody [EPR19814] (ab203486) The Images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University, MARC.
    All lanes : Anti-CSC1 antibody [EPR19814] (ab203486) at 1/400 dilution

    Lane 1 : CSC1 KO (-/-) mouse brain lysates
    Lane 2 : CSC1 WT (+/+) mouse brain lysates
    Lane 3 : CSC1 heterozygous (+/-) mouse brain lysates

    Secondary
    All lanes : Goat Anti-Mouse IgG(H+L) HRP at 1/15000 dilution

    Predicted band size: 93 kDa
    Observed band size: 93 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-CSC1 antibody [EPR19814] (ab203486)
    Immunocytochemistry/ Immunofluorescence - Anti-CSC1 antibody [EPR19814] (ab203486)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HA-mCSC1 transfected HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling CSC1 with ab203486 at 1/150 dilution (green). Counterstained with Anti-HA antibody at 1/500 dilution (red).

    Positive staining on HA-mCSC1 transfected HEK-293T cells. The images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.

    The nuclear counter stain is DAPI (blue).

     

  • Immunoprecipitation - Anti-CSC1 antibody [EPR19814] (ab203486)
    Immunoprecipitation - Anti-CSC1 antibody [EPR19814] (ab203486)

    CSC1 was immunoprecipitated from 0.35 mg of mouse cerebellum lysate with ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse cerebellum lysate 10 μg (Input).

    Lane 2: ab203486 IP in mouse cerebellum lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203486 in mouse cerebellum lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    Note: The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates.

  • Immunoprecipitation - Anti-CSC1 antibody [EPR19814] (ab203486)
    Immunoprecipitation - Anti-CSC1 antibody [EPR19814] (ab203486)

    CSC1 was immunoprecipitated from 0.35 mg of rat cerebellum lysate with ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Rat cerebellum lysate 10 μg (Input).

    Lane 2: ab203486 IP in rat cerebellum lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203486 in rat cerebellum lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    Note: The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates.

  • Anti-CSC1 antibody [EPR19814] (ab203486)
    Anti-CSC1 antibody [EPR19814] (ab203486)

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