Anti-Superoxide Dismutase 3/EC-SOD antibody (ab90258)
Key features and details
- Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Superoxide Dismutase 3/EC-SOD antibody
See all Superoxide Dismutase 3/EC-SOD primary antibodies -
Description
Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following human tissue lysates: Artery; Heart; Lung; Kidney.
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General notes
Previously labelled as Superoxide Dismutase 3.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).Target
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Function
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. -
Tissue specificity
Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. -
Sequence similarities
Belongs to the Cu-Zn superoxide dismutase family. -
Cellular localization
Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium. - Information by UniProt
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Database links
- Entrez Gene: 6649 Human
- Omim: 185490 Human
- SwissProt: P08294 Human
- Unigene: 2420 Human
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Alternative names
- EC SOD antibody
- EC-SOD antibody
- Extracellular superoxide dismutase [Cu Zn] antibody
see all
Images
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Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab90258)All lanes : Anti-Superoxide Dismutase 3/EC-SOD antibody (ab90258) at 1 µg/ml
Lane 1 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Lung (Human) Tissue Lysate
Lane 4 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 32,35 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 42 kDa, 50 kDa, 55 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesSuperoxide dismutase 3 / EC-SOD contains a number of potential glycosylation sites (SwissProt), which may explain the migration pattern observed.
References (1)
ab90258 has been referenced in 1 publication.
- Katamune C et al. Mutation of the gene encoding the circadian clock component PERIOD2 in oncogenic cells confers chemoresistance by up-regulating the Aldh3a1 gene. J Biol Chem 294:547-558 (2019). PubMed: 30429219
Images
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Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab90258)All lanes : Anti-Superoxide Dismutase 3/EC-SOD antibody (ab90258) at 1 µg/ml
Lane 1 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Lung (Human) Tissue Lysate
Lane 4 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 32,35 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 42 kDa, 50 kDa, 55 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesSuperoxide dismutase 3 / EC-SOD contains a number of potential glycosylation sites (SwissProt), which may explain the migration pattern observed.
