Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)
![Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)](https://www.abcam.com/ps/products/80/ab80946/Images/ab80946-96192-anti-superoxide-dismutase-3-ec-sod-antibody-4g11g6-western-blot.jpg "Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)")
Key features and details
- Mouse monoclonal [4G11G6] to Superoxide Dismutase 3/EC-SOD
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6]
See all Superoxide Dismutase 3/EC-SOD primary antibodies -
Description
Mouse monoclonal [4G11G6] to Superoxide Dismutase 3/EC-SOD -
Host species
Mouse -
Specificity
Detects extracellular Superoxide Dismutase 3/EC-SOD.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Mouse
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Immunogen
Full length native protein (purified) corresponding to Human Superoxide Dismutase 3/EC-SOD.
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Positive control
- Human cartilage lysate
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General notes
Previously labelled as Superoxide Dismutase 3.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4G11G6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946) at 1 µg/ml + Lung (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)](https://www.abcam.com/ps/products/80/ab80946/Images/ab80946-96193-anti-superoxide-dismutase-3-ec-sod-antibody-4g11g6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)ab80946 at 1/100 dilution staining Superoxide Dismutase 3/EC-SOD in human cartilage by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP-conjugated anti mouse secondary was used at 1/10 dilution. Image 'a' repersents control, 'b' staining of tissue from young human cartilage and 'c' show staining cartilage of a person suffered from osteoarthritis.
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![Flow Cytometry - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)](https://www.abcam.com/ps/products/80/ab80946/Images/ab80946-96191-anti-superoxide-dismutase-3-ec-sod-antibody-4g11g6-flow-cytometry.jpg)
Flow Cytometry - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946)Overlay histogram showing HEK293 cells stained with ab80946 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80946, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
