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Cell Biology Other Antibodies Oxidative Stress

Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)

Price and availability

284 784 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD
  • Suitable for: IHC-Fr, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Superoxide Dismutase 3/EC-SOD antibody
    See all Superoxide Dismutase 3/EC-SOD primary antibodies
  • Description

    Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD
  • Host species

    Rabbit
  • Specificity

    Specific to human extracellular SOD (SOD3). Under reducing conditions the SOD3 antibody shows a major band at approximately 30kDa which is the approximate molecular weight of the SOD3 monomer and two faint minor bands at approximately 50 and 70 kDa. The SOD3 molecule is a homotetramer (4 monomers) with a combined molecular weight of around a 130 Kda. The two faint bands are probably dimers and triplicates of the monomer. This pattern is common with many tetramers (such as cellular glutathione peroxidase) as there is incomplete reduction with DTT.
  • Tested applications

    Suitable for: IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Superoxide Dismutase 3/EC-SOD (N terminal).
    Sequence:

    WTGEDSAEPNSDSAEWIRD

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC: cortex of Dementia patient with Lewy bodies WB: human lung lysate
  • General notes

    Previously labelled as Superoxide Dismutase 3.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Thimerosal (merthiolate)
    Constituent: Whole serum
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)
    Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)
    ab21974 (1/1000) staining of ciliated bronchiolar cells in human lung.
  • Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)
    Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)
    ab21974 (1/1000) staining of human kidney tubule cells.
  • Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)
    Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)
    All lanes : Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974) at 1/500 dilution

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 31 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    Superoxide Dismutase 3 / EC-SOD contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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