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Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK293T cell pellet (ab278982)

Overview

  • Product name

    Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK293T cell pellet
    See all Peroxiredoxin 1/PAG kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.

  • Sequence similarities

    Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.

  • Post-translational
    modifications

    Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.

  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Alternative names

    • Heme binding 23 kDa protein
    • MSP23
    • Natural killer cell-enhancing factor A
    • NKEF A
    • NKEF-A
    • NKEFA
    • OSF3
    • Osteoblast specific factor 3
    • PAG
    • Paga
    • PAGB
    • Peroxiredoxin 1
    • Peroxiredoxin-1
    • PRDX1
    • PRDX1_HUMAN
    • Proliferation associated gene A
    • Proliferation-associated gene protein
    • PRX1
    • PrxI
    • TDPX2
    • Thioredoxin peroxidase 2
    • Thioredoxin-dependent peroxide reductase 2
    see all

Properties

Images

  • Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK293T cell pellet (ab278982)
    Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK293T cell pellet (ab278982)

    Lane 1:Wild-type HEK293T cell lysate (20 ug)
    Lane 2:PRDX1 knockout HEK293T cell lysate (20 ug)
    Lane 3:Jurkat cell lysate (20 ug)

    ab109506 was shown to specifically react with Peroxiredoxin 1/PAG in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266842 (knockout cell lysate ab257040) was used. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab109506 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK293T cell pellet (ab278982)
    Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK293T cell pellet (ab278982)

    Lane 1:Wild-type HEK293T cell lysate (20 ug)
    Lane 2:PRDX1 knockout HEK293T cell lysate (20 ug)
    Lane 3:Jurkat cell lysate (20 ug)

    ab109498 was shown to specifically react with Peroxiredoxin 1/PAG in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266842 (knockout cell lysate ab257040) was used. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab109498 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK293T cell pellet (ab278982)
    Sanger Sequencing - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK293T cell pellet (ab278982)
    Homozygous: 1 bp deletion in exon2

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