Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab105731)
![Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab105731)](https://www.abcam.com/ps/products/105/ab105731/Images/ab105731-118154-anti-akt1-phospho-t308-antibody-18f3h11-western-blot.jpg "Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab105731)")
Key features and details
- Mouse monoclonal [18F3.H11] to AKT1 (phospho T308)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-AKT1 (phospho T308) antibody [18F3.H11]
See all AKT1 primary antibodies -
Description
Mouse monoclonal [18F3.H11] to AKT1 (phospho T308) -
Host species
Mouse -
Specificity
Specific for AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Vertebrata
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Immunogen
Synthetic peptide corresponding to Human AKT1 (internal sequence) (phospho T308).
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Positive control
- PDGF stimulated NIH/3T3 cell lysates; Human brain cerebellum tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from concentrated tissue culture supernate. -
Clonality
Monoclonal -
Clone number
18F3.H11 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab105731)All lanes : Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab105731) at 1/4000 dilution
Lane 1 : Unstimulated NIH/3T3 cell lysates
Lane 2 : PDGF stimulated NIH/3T3 cell lysates
Lysates/proteins at 15 µg per lane.
Predicted band size: 55 kDa
Gel concentration: 4-20% -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab105731)](https://www.abcam.com/ps/products/105/ab105731/Images/ab105731-118153-anti-akt1-phospho-t308-antibody-18f3h11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab105731)ab105731, at 20 µg/ml, staining AKT1 (phospho T308) in formalin fixed, praffin embedded Human brain cerebellum tissue (40X) by Immunohistochemistry.
The image shows strong staining of Purkinje neurons and cell processes in the cerebellum. Staining was both cytosolic as well as occasionally nuclear, and ab105731 showed minimal background staining. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
