Anti-AKT2 (phospho S474) antibody (ab38513)
Key features and details
- Rabbit polyclonal to AKT2 (phospho S474)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AKT2 (phospho S474) antibody
See all AKT2 primary antibodies -
Description
Rabbit polyclonal to AKT2 (phospho S474) -
Host species
Rabbit -
Specificity
Akt2 (phospho-Ser474) antibody detects endogenous levels of Akt2 only when phosphorylated at serine 474. The region of AKT2 surrounding S474 has a high degree of similarity to the corresponding regions in AKT1 and AKT3 and thus may cross react with these proteins if phosphorylated on the corresponding serine residue. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human AKT2 aa 432-481 (C terminal) (phospho S474). The exact sequence is proprietary. The antiserum was produced against synthesized phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474.
Sequence:SEVDTRYFD DEFTAQSITI TPPDRYDSLG LLELDQRTHF PQFSYSASIR E
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AKT2 (phospho S474) antibody (ab38513)All lanes : Anti-AKT2 (phospho S474) antibody (ab38513)
Lane 1 : 293 cells treated for 30 min with 200ng/ml EGF
Lane 2 : 293 cells treated for 30 min with 200ng/ml EGF with Phospho peptide
Predicted band size: 55 kDa
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Western blot - Anti-AKT2 (phospho S474) antibody (ab38513)All lanes : Anti-AKT2 (phospho S474) antibody (ab38513) at 1/500 dilution
Lane 1 : Extract of A2780 cells.
Lane 2 : Extract of A2780 cells + TNF alpha.
Lane 3 : Extract of A2780 cells + UV.
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)
Predicted band size: 55 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Lanes can be loaded with 5-30µg of total protein. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT2 (phospho S474) antibody (ab38513)This image shows human lung carcinoma tissue stained with ab38513 at 1/50 dilution.
Image on left: untreated.
Image on right: treated with phosphopeptide at 1µg/ml (negative control).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT2 (phospho S474) antibody (ab38513) This image is courtesy of an anonymous Abreviewab38513 staining AKT2 in human infantile fibromatosis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% FBS/BSA for 3 hours at room temperature; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/150 in TBS + 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
