Anti-AKT2 antibody (ab223617)
Key features and details
- Rabbit polyclonal to AKT2
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-AKT2 antibody
See all AKT2 primary antibodies -
Description
Rabbit polyclonal to AKT2 -
Host species
Rabbit -
Specificity
ab223617 does not react with AKT1 or AKT3. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB RatHuman
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Immunogen
Synthetic peptide corresponding to Human AKT2 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P31751 -
Positive control
- WB: A43, A549, HCT 116, HeLa, HEK-293, HepG2, HL-60, HUVEC Jurkat, MCF7, PC-3 and T98G cell lysates; Rat brain lysate. ICC/IF: Heat shocked HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), Tris buffered saline -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-AKT2 antibody (ab223617)
2% formaledehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for AKT2 (green) using ab223617 at 1/25 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit FITC at 1/200 dilution for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at room temperature.
(A) DAPI (blue) nuclear stain. (B) ab223617. (C) Merge.
Cells heat shocked at 42°C for 1 hour.
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Western blot - Anti-AKT2 antibody (ab223617)All lanes : Anti-AKT2 antibody (ab223617) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : A549 (human lung carcinoma cell line) cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 7 : HL-60 (human promyelocytic leukemia cell line) cell lysate
Lane 8 : HUVEC (human umbilical vein endothelial cell line) cell lysate
Lane 9 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 10 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 11 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Lane 12 : T98G cell lysate
Lane 13 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Donkey Anti-Rabbit IgG HRP
Predicted band size: 55 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?Blocking: 1.5% BSA for 30 minutes at room temperature.
