Anti-AKT2 antibody (ab264288)
Key features and details
- Rabbit polyclonal to AKT2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AKT2 antibody
See all AKT2 primary antibodies -
Description
Rabbit polyclonal to AKT2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human AKT2 aa 431-481. The exact sequence is proprietary.
Database link: P31751 -
Positive control
- WB: HEK-293T, HeLa and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AKT2 antibody (ab264288)All lanes : Anti-AKT2 antibody (ab264288) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Exposure time: 3 seconds4-20% SDS-PAGE.
Cells prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-AKT2 antibody (ab264288)AKT2 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264288 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264288 at 1 µg/ml.
Lane 1: ab264288 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 secs.
Cells prepared using NETN lysis buffer.
