Anti-AKT3 antibody (ab152157)
Key features and details
- Rabbit polyclonal to AKT3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AKT3 antibody
See all AKT3 primary antibodies -
Description
Rabbit polyclonal to AKT3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Recombinant fragment corresponding to Human AKT3 aa 1-164.
Database link: Q9Y243 -
Positive control
- Recombinant Human AKT3 protein (ab43621) can be used as a positive control in WB. Human A549 xenograft tissue; 293T, A431, HepG2, MOLT4, Raji, HA-AKT transfected HeLa cells and mouse brain whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AKT3 antibody (ab152157)All lanes : Anti-AKT3 antibody (ab152157) at 1/1000 dilution
Lane 1 : Non-transfected 293T whole cell extracts
Lane 2 : Transfected (+) 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 antibody (ab152157)Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue, staining AKT3 using ab152157 at a 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-AKT3 antibody (ab152157)Immunofluorescent analysis of methanol fixed MCF-7 cells labeling AKT3 with ab152157 at 1 in 500 dilution.
Blue: Hoechst 33342 staining.
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Western blot - Anti-AKT3 antibody (ab152157)Anti-AKT3 antibody (ab152157) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS PAGE -
Western blot - Anti-AKT3 antibody (ab152157)Anti-AKT3 antibody (ab152157) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 54 kDa
7.5% SDS PAGE
