Recombinant human AKT3 (mutated E17K) protein (ab186457)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human AKT3 (mutated E17K) protein
See all AKT3 proteins and peptides -
Biological activity
The specific activity of ab186457 was determined to be 480 nmol/min/mg as per activity assay protocol.
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Purity
> 95 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSDVTIVKEGWVQKRGKYIKNWRPRYFLLKTDGSFIGYKEKPQDVDLPYP LNNFSVAKCQLMKTERPKPNTFIIRCLQWTTVIERTFHVDTPEEREEWTE AIQAVADRLQRQEEERMNCSPTSQIDNIGEEEMDASTTHHKRKTMNDFDY LKLLGKGTFGKVILVREKASGKYYAMKILKKEVIIAKDEVAHTLTESRVL KNTRHPFLTSLKYSFQTKDRLCFVMEYVNGGELFFHLSRERVFSEDRTRF YGAEIVSALDYLHSGKIVYRDLKLENLMLDKDGHIKITDFGLCKEGITDA ATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQD HEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDAKEIM RHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPE KYDEDGMDCMDNERRPHFPQFSYSASGRE -
Predicted molecular weight
83 kDa including tags -
Amino acids
1 to 479 -
Modifications
mutated E17K -
Tags
GST tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.88% Sodium chloride, 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.003% EDTA, 0.004% DTT, 0.31% Glutathione, 0.002% PMSFThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human AKT3 (mutated E17K) protein (ab186457)
Specific activity of ab186457 was determined to be 480 nmol/min/mg as per activity assay protocol.
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SDS-PAGE - Recombinant human AKT3 (mutated E17K) protein (ab186457)Purity of ab186457 was determined to be >95% by densitometry
