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Recombinant human AKT3 (mutated G171R) protein (ab177263)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 95% Densitometry
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: SDS-PAGE, Functional Studies, WB

Description

  • Product name

    Recombinant human AKT3 (mutated G171R) protein
    See all AKT3 proteins and peptides

  • Biological activity

    140 nmol/min/mg

  • Purity

    > 95 % Densitometry.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    Q9Y243

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Predicted molecular weight

      85 kDa including tags

    • Amino acids

      1 to 479

    • Modifications

      mutated G171R

    • Tags

      GST tag N-Terminus

Preparation and Storage

  • Alternative names

    • Akt3
    • AKT3 kinase
    • AKT3_HUMAN
    • DKFZp434N0250
    • MPPH
    • PKB gamma
    • PKBG
    • PRKBG
    • Protein kinase Akt-3
    • Protein Kinase AKT3
    • Protein kinase B gamma
    • RAC gamma
    • RAC gamma serine/threonine protein kinase
    • RAC-gamma serine/threonine-protein kinase
    • RAC-PK-gamma
    • RACPK Gamma
    • Serine threonine protein kinase Akt 3
    • Serine threonine protein kinase Akt3
    • STK 2
    • STK-2
    • STK2
    • V akt murine thymoma viral oncogene homolog 3
    • V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
    • V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma
    see all

  • Function

    IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.

  • Tissue specificity

    In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.

  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.

  • Domain

    Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.

  • Post-translational
    modifications

    Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.

  • Cellular localization

    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human AKT3 (mutated G171R) protein (ab177263)
    Functional Studies - Recombinant human AKT3 (mutated G171R) protein (ab177263)

    The specific activity of ab177263 was determined to be 140 nmol /min/mg.

     

  • SDS-PAGE - Recombinant human AKT3 (mutated G171R) protein (ab177263)
    SDS-PAGE - Recombinant human AKT3 (mutated G171R) protein (ab177263)

    SDS-PAGE analysis of ab177263.

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