Recombinant human Histone H3 protein (Active) (ab198757)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Histone H3 protein (Active)
See all Histone H3 proteins and peptides -
Biological activity
Assay Conditions: ab198757 was coated onto a 96-well plate, and methylation by G9a was carried out at room temperature for 60 min. Methylated ab198757 was detected using a Chemiluminescence assay.
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Purity
>= 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
ARTKQTARKS TGGKAPRKQL ATKAARKSAP ATGGVKKPHR YRPGTVALRE IRRYQKSTEL LIRKLPFQRL VREIAQDFKT DLRFQSSAVM ALQEACEAYL VGLFEDTNLC AIHAKRVTIM PKDIQLARRI RGERA -
Predicted molecular weight
15 kDa including tags -
Amino acids
2 to 136 -
Tags
His tag N-Terminus -
Additional sequence information
(GenBank NM_003532) Full length mature protein, minus the initiator methionine.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: PBS, 0.64% Sodium chloride, 0.0165% Potassium chloride, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human Histone H3 protein (ab198757)
Methylation of ab198757 by G9a.
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SDS-PAGE - Recombinant human Histone H3 protein (ab198757)2µg ab198757 on 4-20% SDS-PAGE, stained with Coomassie.
MW=16.4 kDa due to glycosalation.
