Antibodies, Proteins, Kits and Reagents for Life Science

Alexa Fluor® 488 Anti-p57 Kip2 antibody [EP2515Y] (ab199069)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Alexa Fluor® 488 Rabbit monoclonal [EP2515Y] to p57 Kip2
Suitable for: Flow Cyt, ICC/IF
Reacts with: Human
Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Product name

Alexa Fluor® 488 Anti-p57 Kip2 antibody [EP2515Y]
See all p57 Kip2 primary antibodies
Description

Alexa Fluor® 488 Rabbit monoclonal [EP2515Y] to p57 Kip2
Host species

Rabbit
Conjugation

Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Tested applications

Suitable for: Flow Cyt, ICC/IFmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC/IF: HeLa and SKNSH cells. Flow Cyt: HeLa cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Alexa Fluor^® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor^® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor^® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor^® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP2515Y
Isotype

IgG
Research areas

Flow Cytometry - Alexa Fluor® 488 Anti-p57 Kip2 antibody [EP2515Y] (ab199069)

Overlay histogram showing HeLa cells stained with ab199069 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab199069, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-p57 Kip2 antibody [EP2515Y] (ab199069)

ab199069 staining p57 Kip2 in SKNSH cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199069 at 1/100 dilution (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml. This was followed by an incubation at room temperature for 1h with ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed, at 1µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-p57 Kip2 antibody [EP2515Y] (ab199069)

ab199069 staining p57 Kip2 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199069 at 1/100 dilution (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml. This was followed by an incubation at room temperature for 1h with ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed, at 1µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 488 Anti-p57 Kip2 antibody [EP2515Y] (ab199069)

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