Anti-NFkB p105 / p50 antibody (ab209795)
Key features and details
- Rabbit polyclonal to NFkB p105 / p50
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NFkB p105 / p50 antibody
See all NFkB p105 / p50 primary antibodies -
Description
Rabbit polyclonal to NFkB p105 / p50 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human NFkB p105/ p50 aa 350-550.
Database link: P19838 -
Positive control
- WB: SK-BR-3 and Daudi cell lysates; ICC: A-431 cells; IHC-P: Human lymph node, colon, and urinary bladder tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NFkB p105 / p50 antibody (ab209795)All lanes : Anti-NFkB p105 / p50 antibody (ab209795) at 0.4 µg/ml
Lane 1 : SK-BR-3 (human mammary gland adenocarcinoma cell line) transfected with control siRNA, cell lysate
Lane 2 : SK-BR-3 transfected with target specific siRNA probe #1, cell lysate
Lane 3 : SK-BR-3 transfected with target specific siRNA probe #2, cell lysate
Predicted band size: 105 kDaLoading control: Anti-GAPDH
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody (ab209795)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling NFkB p105 / p50 with ab209795 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry - Anti-NFkB p105 / p50 antibody (ab209795)Immunofluorescent analysis of A431 cells (PFA-fixed/Triton X-100 permeabilized) labeling NFkB p105 / p50 with ab209795 at 4 μg/ml (green).
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Western blot - Anti-NFkB p105 / p50 antibody (ab209795)Anti-NFkB p105 / p50 antibody (ab209795) at 0.4 µg/ml + Daudi (human Burkitt's lymphoma cell line) cell lysate
Predicted band size: 105 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody (ab209795)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human urinary bladder tissue labelling NFkB p105 / p50 with ab209795 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody (ab209795)Immunohistochemical analysis of Paraffin-embedded Human lymph node tissue labeling NFkB p105 / p50 with ab209795 at 1/200 dilution.
