HRP Anti-NFkB p105 / p50 antibody [E381] (ab195854)
![HRP Anti-NFkB p105 / p50 antibody [E381] (ab195854)](https://www.abcam.com/ps/products/195/ab195854/Images/ab195854-312625-anti-nfkb-p105-p50-antibody-e381-hrp-western-blot.jpg "HRP Anti-NFkB p105 / p50 antibody [E381] (ab195854)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [E381] to NFkB p105 / p50
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-NFkB p105 / p50 antibody [E381]
See all NFkB p105 / p50 primary antibodies -
Description
HRP Rabbit monoclonal [E381] to NFkB p105 / p50 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E381 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-NFkB p105 / p50 antibody [E381] (ab195854)All lanes : HRP Anti-NFkB p105 / p50 antibody [E381] (ab195854) at 1/2000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : Nfkb1 (NFkB p105 / p50) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 50 kDa
Observed band size: 105,50 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesab195854 was shown to recognize NFkB p105 / p50 in wild-type HAP1 cells as signal was lost at the expected MW in Nfkb1 (NFkB p105 / p50) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Nfkb1 (NFkB p105 / p50) knockout samples were subjected to SDS-PAGE. Ab195854 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-NFkB p105 / p50 antibody [E381] (ab195854)](https://www.abcam.com/ps/products/195/ab195854/Images/ab195854-241888-anti-nfkb-p105-p50-antibody-e381-hrp-western-blot.jpg)
Western blot - HRP Anti-NFkB p105 / p50 antibody [E381] (ab195854)HRP Anti-NFkB p105 / p50 antibody [E381] (ab195854) at 1/2000 dilution +HeLa whole cell lysate (ab150035) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 105,50 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195854 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-NFkB p105 / p50 antibody [E381] (ab195854)](https://www.abcam.com/ps/products/195/ab195854/Images/ab195854-3-benefits-of-recombinant-antibodies.png)
HRP Anti-NFkB p105 / p50 antibody [E381] (ab195854)
