CIC-C3d ELISA Kit (ab178665)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
CIC-C3d ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 16 6.1% Inter-assay Sample n Mean SD CV% Serum 13.9% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s CIC-C3d in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of CIC-C3d in Human serum and plasma.
A 96-well plate has been precoated with anti-C3d antibodies. Standards, controls and test samples are added to the wells and incubated. Immune complexes (CIC) in the samples and standards bind to the precoated antibody. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized CIC-C3d. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of CIC-C3d in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Solution 2 x 50ml Anti-CIC-C3d Coated Microplate (12 x 8 wells) 1 unit CIC-C3d Anti-IgG HRP Conjugate 1 x 0.5ml CIC-C3d Negative Control 1 x 1.5ml CIC-C3d Positive Control 1 x 1.5ml CIC-C3d Standard 0 - 0 µg Eq/mL 1 x 1.5ml CIC-C3d Standard 1 - 16 µg Eq/mL 1 x 1.5ml CIC-C3d Standard 2 - 64 µg Eq/mL 1 x 1.5ml Conjugate Buffer 1 x 20ml Cover Foil 1 unit Incubation Buffer 1 x 50ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
Research areas
Images
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Typical Standard Curve
Representative standard curve using ab178665.
