Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3980] to Annexin V/ANXA5
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Annexin V/ANXA5 antibody [EPR3980]
    See all Annexin V/ANXA5 primary antibodies

  • Description

    Rabbit monoclonal [EPR3980] to Annexin V/ANXA5

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 80% homology with ANXA2 and 73% homology with ANXA4. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, fetal kidney, fetal brain, Jurkat and JAR cell lysates ICC: HeLa cells; staurosporin-treated apoptotic HeLa cells

  • General notes

     This product was previously labelled as Annexin V

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Annexin V knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab108194 observed at 35 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab108194 was shown to recognize Annexin V in wild-type cells as signal was lost at the expected MW in Annexin V knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Annexin V knockout samples were subjected to SDS-PAGE. Ab108194 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Flow Cytometry - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Overlay histogram showing HeLa cells stained with ab108194 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108194, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    All lanes : Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)

    Lane 1 : HeLa cell lysate
    Lane 2 : Fetal kidney lysate
    Lane 3 : Fetal brain lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : JAR cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 36 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Immunofluorescent staining of HeLa cells using 1/500 ab108194.
  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Immunofluorescent staining of staurosporin-treated apoptotic HeLa cells using 1/250 ab108194.
  • Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
    Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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