Anti-Annexin V/ANXA5 antibody (ab137823)
Key features and details
- Rabbit polyclonal to Annexin V/ANXA5
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Annexin V/ANXA5 antibody
See all Annexin V/ANXA5 primary antibodies -
Description
Rabbit polyclonal to Annexin V/ANXA5 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human Annexin V/ANXA5 aa 70-285.
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji and mouse brain whole cell lysates Flow cyt: HeLa cells.
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General notes
This product was previously labelled as Annexin V
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Annexin V/ANXA5 antibody (ab137823) at 1/5000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : Annexin V/ANXA5 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 35 kDa
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Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Annexin V/ANXA5 using ab137823 at 1/50 dilution (red) compared to an unlabeled control (black).
A Dylight 488 secondary antibody was used.
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All lanes : Anti-Annexin V/ANXA5 antibody (ab137823) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
10% SDS PAGE -
Anti-Annexin V/ANXA5 antibody (ab137823) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 35 kDa
10% SDS PAGE