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Anti-Annexin V/ANXA5 antibody (ab137823)

Anti-Annexin V/ANXA5 antibody (ab137823)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Annexin V/ANXA5
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Annexin V/ANXA5 antibody
    See all Annexin V/ANXA5 primary antibodies
  • Description

    Rabbit polyclonal to Annexin V/ANXA5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human Annexin V/ANXA5 aa 70-285.

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji and mouse brain whole cell lysates Flow cyt: HeLa cells.
  • General notes

     This product was previously labelled as Annexin V

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Annexin V Reagents
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Detection kits
    • Annexin V
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Apoptosis marker and proteins ELISA kits
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Annexin V
    • Annexin V Detection Kits
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Annexin V
    • Annexin V Reagents

Images

  • Western blot - Anti-Annexin V/ANXA5 antibody (ab137823)
    Western blot - Anti-Annexin V/ANXA5 antibody (ab137823)
    All lanes : Anti-Annexin V/ANXA5 antibody (ab137823) at 1/5000 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : Annexin V/ANXA5 Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 35 kDa

  • Flow Cytometry - Anti-Annexin V/ANXA5 antibody (ab137823)
    Flow Cytometry - Anti-Annexin V/ANXA5 antibody (ab137823)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Annexin V/ANXA5 using ab137823 at 1/50 dilution (red) compared to an unlabeled control (black).

    A Dylight 488 secondary antibody was used.

  • Western blot - Anti-Annexin V/ANXA5 antibody (ab137823)
    Western blot - Anti-Annexin V/ANXA5 antibody (ab137823)
    All lanes : Anti-Annexin V/ANXA5 antibody (ab137823) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    10% SDS PAGE
  • Western blot - Anti-Annexin V/ANXA5 antibody (ab137823)
    Western blot - Anti-Annexin V/ANXA5 antibody (ab137823)
    Anti-Annexin V/ANXA5 antibody (ab137823) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 35 kDa



    10% SDS PAGE

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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