Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SET/TAF-I antibody [EPR12973] (ab181990)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12973] to SET/TAF-I
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-SET/TAF-I antibody [EPR12973]
    See all SET/TAF-I primary antibodies

  • Description

    Rabbit monoclonal [EPR12973] to SET/TAF-I

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat , 293T, HeLa, HepG2 whole cell lysate (ab7900); Human nephroblastoma and rat spleen tissues.

  • General notes

    This product was previously labelled as SET

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Western blot - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    All lanes : Anti-SET/TAF-I antibody [EPR12973] (ab181990) at 1/100000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Hela cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa



    Blocking/dilution buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling SET/TAF-I with Purified ab181990 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)

    Immunohistochemical analysis of paraffin-embedded Human nephroblastoma tissue labeling SET/TAF-I with ab181990 at a 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling SET/TAF-I with ab181990 at a 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cell line staining SET/TAF-I using ab181990 at 1/1000 dilution. A Dylight 555 conjugated Goat anti rabbit IgG at 1/200 dilution was used for secondary antibody detection. Counter stain: Dapi.

  • Flow Cytometry - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Flow Cytometry - Anti-SET/TAF-I antibody [EPR12973] (ab181990)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Hela cells labeling SET/TAF-I using ab181990 at a 1/10 dilution, or a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.

  • Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12973] (ab181990)

    Immunoprecipitation of Jurkat cell lysate labeling SET/TAF-I using ab181990 at a 1/30 dilution (lane 1). A goat anti-rabbit IgG, (H+L), peroxidase conjugated secondary antibody was used at a dilution of 1/1000. Blocking/dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Anti-SET/TAF-I antibody [EPR12973] (ab181990)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-SET/TAF-I antibody [EPR12973] (ab181990)

© 2021 Astana Biomed Group LLP. All rights reserved.