Antibodies, Proteins, Kits and Reagents for Life Science

2 010 ₸ Product size

1 ml 2 010 ₸ 100 µl 278 083 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CD8 alpha antibody [CAL38] (ab237723)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [CAL38] to CD8 alpha
Suitable for: IHC-Fr, IP, IHC-P
Reacts with: Mouse

Product name

Anti-CD8 alpha antibody [CAL38]
See all CD8 alpha primary antibodies
Description

Rabbit monoclonal [CAL38] to CD8 alpha
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse
IP	Mouse

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Mouse spleen tissue. IHC-Fr: Mouse Thymus tissue. IP: Mouse thymus tissue lysate.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

Purity >99%.
Clonality

Monoclonal
Clone number

CAL38
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL38] (ab237723)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD8 alpha with ab237723 at 1/500 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on the mouse spleen is shown. The section was incubated with ab237723 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Frozen sections) - Anti-CD8 alpha antibody [CAL38] (ab237723)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse thymus tissue labeling CD8 alpha with ab237723 at 1/250 dilution, follwed by a AlexaFluor^®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution. Positive staining on the membrane of T cells in mouse thymus (PMID: 29042640) is observed. Counterstained with DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a AlexaFluor^®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution.
Immunoprecipitation - Anti-CD8 alpha antibody [CAL38] (ab237723)

CD8 alpha was immunoprecipitated from 0.35 mg of Mouse thymus tissue lysate with ab237723 at 1/20 dilution. Western blot was performed on the immunoprecipitate using ab237723 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

Lane 1: Mouse thymus tissue lysate 10µg (input)
Lane 2: ab237723 IP in mouse thymus tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237723 in Mouse thymus lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL38] (ab237723)

Formalin-fixed, paraffin-embedded mouse spleen tissue stained for CD8 alpha with ab237723 at 0.125 µg/ml in immunohistochemical analysis.
Anti-CD8 alpha antibody [CAL38] (ab237723)

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