Anti-METTL14 antibody (ab252562)
Key features and details
- Rabbit polyclonal to METTL14
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-METTL14 antibody
See all METTL14 primary antibodies -
Description
Rabbit polyclonal to METTL14 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Xenopus laevis, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide within Human METTL14 aa 25-75. The exact sequence is proprietary.
Database link: Q9HCE5 -
Positive control
- WB: Jurkat, RKO, GaMG and TCMK-1 whole cell lysate. IP: Jurkat whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab252562 was affinity purified using an epitope specific to METTL14 immobilized on a solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-METTL14 antibody (ab252562)All lanes : Anti-METTL14 antibody (ab252562) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : RKO whole cell lysate
Lane 3 : GaMG whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 52 kDa
Exposure time: 10 secondsLysates prepared in NETN buffer.
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Immunoprecipitation - Anti-METTL14 antibody (ab252562)METTL14 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab252562 at 100 μL per reaction. Western blot was performed from the immunoprecipitate using ab252562 at 1/1000 dilution.
Lane 1: ab252562 IP in Jurkat whole cell lysate.
Lane 2: ab264408 instead of ab252562 in Jurkat whole cell lysate.
Lane 3: Control IgG IP in Jurkat whole cell lysate.Detection: Chemiluminescence with exposure time of 3 seconds.
Lysates prepared in NETN buffer.
