Anti-GSDMD antibody - C-terminal (ab228824)
Key features and details
- Rabbit polyclonal to GSDMD - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GSDMD antibody - C-terminal
See all GSDMD primary antibodies -
Description
Rabbit polyclonal to GSDMD - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human GSDMD aa 434-484 (C terminal). The exact sequence is proprietary.
Database link: P57764 -
Positive control
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab228824 was affinity purified using an epitope specific to GSDMD immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-GSDMD antibody - C-terminal (ab228824)
GSDMD was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab228824 at 20 µl per reaction. Western blot was performed from the immunoprecipitate using ab228824 at 1/100 dilution.
Lane 1: ab228824 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.Detection:Chemiluminescence with exposure time of 30 seconds.
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Western blot - Anti-GSDMD antibody - C-terminal (ab228824)All lanes : Anti-GSDMD antibody - C-terminal (ab228824) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 52 kDa
Exposure time: 3 minutesLysates were prepared using NETN lysis buffer.
