Anti-GSDMD antibody [EPR19829] - BSA and Azide free (ab232485)
![Anti-GSDMD antibody [EPR19829] - BSA and Azide free (ab232485)](https://www.abcam.com/ps/products/232/ab232485/Images/ab232485-308958-anti-gsdmd-antibody-epr19829-western-blot.jpg "Anti-GSDMD antibody [EPR19829] - BSA and Azide free (ab232485)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19829] to GSDMD - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-GSDMD antibody [EPR19829] - BSA and Azide free
See all GSDMD primary antibodies -
Description
Rabbit monoclonal [EPR19829] to GSDMD - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa whole cell lysate.
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General notes
Ab232485 is the carrier-free version of ab210070. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232485 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19829 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GSDMD antibody [EPR19829] - BSA and Azide free (ab232485)All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : GSDMD knockout HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The cell lysates were kindly provided by our collaborator Dr. Feng Shao, NIBS.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210070).
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![Anti-GSDMD antibody [EPR19829] - BSA and Azide free (ab232485)](https://www.abcam.com/ps/products/232/ab232485/Images/ab232485-2-benefits-of-recombinant-antibodies.png)
Anti-GSDMD antibody [EPR19829] - BSA and Azide free (ab232485)
