Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GSDMD antibody [EPR19829] (ab210070)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19829] to GSDMD
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-GSDMD antibody [EPR19829]
    See all GSDMD primary antibodies

  • Description

    Rabbit monoclonal [EPR19829] to GSDMD

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant Human GSDMD protein (ab164113), HeLa, 293T, PC-3, A549 , SH-SY5Y and Jurkat whole cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19829

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GSDMD antibody [EPR19829] (ab210070)
    Western blot - Anti-GSDMD antibody [EPR19829] (ab210070)
    All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution

    Lane 1 : THP-1 (Human monocytic leukemia monocyte) cells were differentiated into macrophage-like cells by incubation in the presence of 50ng/ul PMA for 48h. Then incubated for 90 min without treatment whole cell lysate.
    Lane 2 : THP-1 (Human monocytic leukemia monocyte) cells were differentiated into macrophage-like cells by incubation in the presence of 50ng/ul PMA for 48h. Then treated with PrgI and PA (a NAIP-NLRC4 inflammasome trigger) for 90 min whole cell lysate.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 23,53 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking and diluting buffer was 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator, Dr. Feng Shao, NIBS.

    The 43 kDa band is likely to be a GSDMD cleavage product by caspase-3 at GSDMD Asp88 (PMID: 30902848, PMID: 28392147 , PMID: 30135078).

  • Western blot - Anti-GSDMD antibody [EPR19829] (ab210070)
    Western blot - Anti-GSDMD antibody [EPR19829] (ab210070)
    All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution

    Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
    Lane 5 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

  • Western blot - Anti-GSDMD antibody [EPR19829] (ab210070)
    Western blot - Anti-GSDMD antibody [EPR19829] (ab210070)
    All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : Human Gasdermin-D knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator Dr. Feng Shao, NIBS.

  • Western blot - Anti-GSDMD antibody [EPR19829] (ab210070)
    Western blot - Anti-GSDMD antibody [EPR19829] (ab210070)
    All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution

    Lane 1 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Anti-GSDMD antibody [EPR19829] (ab210070)
    Anti-GSDMD antibody [EPR19829] (ab210070)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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