Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µl 324 988 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GSDMD antibody [EPR19829] (ab210070)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19829] to GSDMD
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-GSDMD antibody [EPR19829]
See all GSDMD primary antibodies
Description

Rabbit monoclonal [EPR19829] to GSDMD
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Recombinant Human GSDMD protein (ab164113), HeLa, 293T, PC-3, A549 , SH-SY5Y and Jurkat whole cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19829
Isotype

IgG
Research areas

Western blot - Anti-GSDMD antibody [EPR19829] (ab210070)

All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution

Lane 1 : THP-1 (Human monocytic leukemia monocyte) cells were differentiated into macrophage-like cells by incubation in the presence of 50ng/ul PMA for 48h. Then incubated for 90 min without treatment whole cell lysate.
Lane 2 : THP-1 (Human monocytic leukemia monocyte) cells were differentiated into macrophage-like cells by incubation in the presence of 50ng/ul PMA for 48h. Then treated with PrgI and PA (a NAIP-NLRC4 inflammasome trigger) for 90 min whole cell lysate.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 53 kDa
Observed band size: 23,53 kDa
why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking and diluting buffer was 5% NFDM/TBST.

The cell lysates were kindly provided by our collaborator, Dr. Feng Shao, NIBS.

The 43 kDa band is likely to be a GSDMD cleavage product by caspase-3 at GSDMD Asp88 (PMID: 30902848, PMID: 28392147 , PMID: 30135078).
Western blot - Anti-GSDMD antibody [EPR19829] (ab210070)

All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution

Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 5 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Exposure time: 3 minutes

Blocking/Diluting buffer and concentration 5% NFDM/TBST
Western blot - Anti-GSDMD antibody [EPR19829] (ab210070)

All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution

Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Human Gasdermin-D knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The cell lysates were kindly provided by our collaborator Dr. Feng Shao, NIBS.
Western blot - Anti-GSDMD antibody [EPR19829] (ab210070)

All lanes : Anti-GSDMD antibody [EPR19829] (ab210070) at 1/1000 dilution

Lane 1 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-GSDMD antibody [EPR19829] (ab210070)

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