Anti-GSDMD antibody [EPR20829-408] - BSA and Azide free (ab255983)
![Anti-GSDMD antibody [EPR20829-408] - BSA and Azide free (ab255983)](https://www.abcam.com/ps/products/255/ab255983/Images/ab255983-349781-anti-cleaved-n-terminal-gsdmd-antibodyepr20829-408-western-blot-a431-human.jpg "Anti-GSDMD antibody [EPR20829-408] - BSA and Azide free (ab255983)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20829-408] to GSDMD - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-GSDMD antibody [EPR20829-408] - BSA and Azide free
See all GSDMD primary antibodies -
Description
Rabbit monoclonal [EPR20829-408] to GSDMD - BSA and Azide free -
Host species
Rabbit -
Specificity
The N-term cleaved Gasdermin-D needs inflammatory caspases in response to canonical and non-canonical inflammasome activators (PubMed: 31548300, PubMed: 27418190, PubMed: 26375259). If need to detect cleavage of GSDMD, please ensure that the samples are treated with inflammation before testing.
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Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Salmonella Infected (MOI:100) A431 whole cell lysate; THP-1 treated with 500 ng/ml EprI for 2h, whole cell lysate.
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General notes
Ab255983 is the carrier-free version of ab215203. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255983 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20829-408 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GSDMD antibody [EPR20829-408] - BSA and Azide free (ab255983)Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203) at 1/1000 dilution + Salmonella Infected (MOI:100) A431 (human epidermoid carcinoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesLysate was kindly provided by Dr Feng Shao.
The molecular weight observed is consistent with literature (PMID: 26375003).
Blocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215203).
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![Anti-GSDMD antibody [EPR20829-408] - BSA and Azide free (ab255983)](https://www.abcam.com/ps/products/255/ab255983/Images/ab255983-2-benefits-of-recombinant-antibodies.png)
Anti-GSDMD antibody [EPR20829-408] - BSA and Azide free (ab255983)
