Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23799-22] to AGO4 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free
    See all AGO4 primary antibodies

  • Description

    Rabbit monoclonal [EPR23799-22] to AGO4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293T, MCF7, THP-1, C2C12, NIH/3T3 and C6 whole cell lysates; Human liver and kidney tissue lysates; Mouse and rat kidney and spleen tissue lysates. IHC-P: Human colon carcinoma tissue; Mouse and rat kidney tissue. IP: THP-1 whole cell lysate.

  • General notes

    ab275966 is the carrier-free version of ab259821. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275966 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)
    Western blot - Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)
    All lanes : Anti-AGO4 antibody [EPR23799-22] (ab259821) at 1/1000 dilution

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human kidney tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat kidney tissue lysate
    Lane 6 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 97 kDa
    Observed band size: 97 kDa



    This data was developed using ab259821, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)

    This data was developed using ab259821, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling AGO4 with ab259821 at 1/100 (5.02 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human colon carcinoma (PMID: 20146808). The section was incubated with ab259821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)
    Immunoprecipitation - Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)

    This data was developed using ab259821, the same antibody clone in a different buffer formulation.

    AGO4 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte), whole cell lysate 10 µg with ab259821 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259821 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: THP-1 whole cell lysate 10 µg

    Lane 2: ab259821 IP in THP-1 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259821 in THP-1 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)
    Western blot - Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)
    All lanes : Anti-AGO4 antibody [EPR23799-22] (ab259821) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 5 : C2C12 (mouse myoblasts myoblast), whole cell lysate
    Lane 6 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 7 : C6 (rat glial tumor glial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 97 kDa
    Observed band size: 97 kDa



    This data was developed using ab259821, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)

    This data was developed using ab259821, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling AGO4 with ab259821 at 1/100 (5.02 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse kidney. The section was incubated with ab259821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)

    This data was developed using ab259821, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling AGO4 with ab259821 at 1/100 (5.02 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat kidney. The section was incubated with ab259821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)
    Anti-AGO4 antibody [EPR23799-22] - BSA and Azide free (ab275966)

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