Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SIRT3 antibody [EPR19755] - BSA and Azide free (ab223531)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19755] to SIRT3 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-SIRT3 antibody [EPR19755] - BSA and Azide free
    See all SIRT3 primary antibodies

  • Description

    Rabbit monoclonal [EPR19755] to SIRT3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, HeLa, HepG2 and HT-29 whole cell lysates; Human fetal brain, fetal heart, fetal kidney and fetal liver lysates. IHC-P: Human liver, hepatocellular carcinoma, colon and colon adenocarcinoma tissues.

  • General notes

    Ab223531 is the carrier-free version of ab217319. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223531 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] - BSA and Azide free (ab223531)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] - BSA and Azide free (ab223531)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling SIRT3 with ab217319 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human liver is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217319).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] - BSA and Azide free (ab223531)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] - BSA and Azide free (ab223531)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling SIRT3 with ab217319 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    According to the literature [PMID: 24774224]: IHC and WB studies both showed a decreased expression of SIRT3 in tumor tissues compared with peritumor tissues. Our IHC-P results also showed decreased expression on about 58% of hepatocellular carcinoma.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217319).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] - BSA and Azide free (ab223531)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] - BSA and Azide free (ab223531)

    Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma tissue labeling SIRT3 with ab217319 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human well-differentiated colon adenocarcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Our IHC-P results show that about 75% of well-differentiated colon carcinoma show increased expression.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217319).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] - BSA and Azide free (ab223531)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] - BSA and Azide free (ab223531)

    Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma tissue labeling SIRT3 with ab217319 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human poor- differentiated colon adenocarcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    According to the literature [PMID: 25105144]: SIRT3 expression level was found to be significantly associated with the lymph node metastasis and tumor stages. Our IHC-P results show that about 25% of poor-differentiated colon carcinoma show decreased expression.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217319).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] - BSA and Azide free (ab223531)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] - BSA and Azide free (ab223531)

    This IHC data was generated using the same anti-SIRT3 antibody clone [EPR19755] in a different buffer formulation (cat# ab217319).

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling SIRT3 with ab217319 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-SIRT3 antibody [EPR19755] - BSA and Azide free (ab223531)
    Anti-SIRT3 antibody [EPR19755] - BSA and Azide free (ab223531)

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