Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)
![Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)](https://www.abcam.com/ps/products/270/ab270523/Images/ab270523-381976-AntiSIRT3-antibody-EPR2351436-immunoprecipitation-mouse-liver.jpg "Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23514-36] to SIRT3 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free
See all SIRT3 primary antibodies -
Description
Rabbit monoclonal [EPR23514-36] to SIRT3 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse heart, liver and kidney tissue lysate; Rat liver and kidney tissue lysate. IP: Mouse and rat liver lysate.
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General notes
ab270523 is the carrier-free version of ab246522. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23514-36 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)
SIRT3 was immunoprecipitated from 0.35 mg mouse liver lysate with ab246522 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246522 at a 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse liver lysate 10μg
Lane 2: ab246522 IP in mouse liver lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246522 in mouse liver lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 secs.
The target band size is very close to IgG light chain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246522).
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![Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)](https://www.abcam.com/ps/products/270/ab270523/Images/ab270523-381977-AntiSIRT3-antibody-EPR2351436-immunoprecipitation-rat-liver.jpg)
Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)SIRT3 was immunoprecipitated from 0.35 mg rat liver lysate with ab246522 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246522 at a 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat liver lysate 10μg
Lane 2: ab246522 IP in rat liver lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246522 in rat liver lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 secs.
The target band size is very close to IgG light chain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246522).
-
![Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)](https://www.abcam.com/ps/products/270/ab270523/Images/ab270523-3-benefits-of-recombinant-antibodies.png)
Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)
