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Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22137] to NKG2A + NKG2E + NKG2C
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137]

  • Description

    Rabbit monoclonal [EPR22137] to NKG2A + NKG2E + NKG2C

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human endometrium WB: HEK-293T cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)

    Immunohistochemical analysis of paraffin-embedded human natural killer-cell lymphoma tissue labeling NKG2A + NKG2E + NKG2C with ab224699 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human natural killer-cell lymphoma is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)
    Western blot - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)
    All lanes : Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699) at 1/1000 dilution

    Lanes 1 & 3 & 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), transfected with an empty vector (vector control), whole cell lysate with 5% NFDM/TBST
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), transfected with human His-tagged NKG2A (aa1-233) expression vector, whole cell lysate with 5% NFDM/TBST
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), transfected with human His-tagged NKG2C (aa1-231) expression vector, whole cell lysate with 5% NFDM/TBST
    Lane 6 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), transfected with human His-tagged NKG2E (aa1-240) expression vector, whole cell lysate. with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 26 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Compared with NKG2A, ab224699 has lower affinity with NKG2C and 2E.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling NKG2A + NKG2E + NKG2C with ab224699 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on a subset of immune cells in human endometrium is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)
    Western blot - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)
    Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699) at 1/1000 dilution + NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa
    Observed band size: 34-38 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Multiple bands are likely due to isoforms and glycosylation. The molecular profile/weight observed is consistent with what has been described in the literature (PMID: 9034158).

  • Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)
    Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)

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