Mouse CD8 alpha ELISA Kit (ab238264)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 46 pg/ml
- Range: 0.31 ng/ml - 20 ng/ml
- Sample type: Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CD8 alpha ELISA Kit
See all CD8 alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Mouse thymus 8 8.8% Inter-assay Sample n Mean SD CV% Mouse thymus 3 5.3% -
Sample type
Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
46 pg/ml -
Range
0.31 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 104 91% - 129% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse CD8 alpha ELISA Kit (ab238264) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD8 alpha protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD8 alpha with 46 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CD8 alpha Capture Antibody 1 x 600µl 10X Mouse CD8 alpha Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Mouse CD8 alpha Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- alpha polypeptide (p32)
- CD_antigen=CD8a
- CD8
see all -
Database links
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
Images
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Other - Mouse CD8 alpha ELISA Kit (ab238264)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse CD8 alpha standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CD8 alpha in mouse thymus extract samples.The concentrations of CD8 alpha were measured in duplicates, interpolated from the CD8 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: thymus 400 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Mouse CD8 alpha ELISA Kit (ab238264)To learn more about the advantages of recombinant antibodies see here.