Mouse CTLA 4 ELISA Kit (ab113346)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 4.92 pg/ml - 1200 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CTLA 4 ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
4.92 pg/ml - 1200 pg/mlRecovery
96 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 76.34 67% - 89% Serum 107.3 101% - 114% Plasma 104.2 100% - 110% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: MouseProduct overview
Abcam’s CTLA4 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CTLA4 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse CTLA4 coated on a 96- well plate. Standards and samples are pipetted into the wells and CTLA4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CTLA4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CTLA4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
MicroplateProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse CTLA4 2 vials CTLA4 Microplate (12 strips x 8 wells) 1 unit Recombinant Mouse CTLA4 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse CTLA4 2 vials CTLA4 Microplate (12 strips x 8 wells) 1 unit Recombinant Mouse CTLA4 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
Images
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Representative Standard Curve
Representative Standard Curve using ab113346
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Representative Standard CurveRepresentative Standard Curve using ab113346