Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NCR1 antibody [EPR22403-57] (ab224703)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22403-57] to NCR1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-NCR1 antibody [EPR22403-57]
    See all NCR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22403-57] to NCR1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NK-92 whole cell lysate. IHC-P: Human tonsil and NK/T cell lymphoma.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22403-57

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR22403-57] (ab224703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR22403-57] (ab224703)

    Immunohistochemical analysis of paraffin-embedded human NK/T cell lymphoma tissue labeling NCR1 with ab224703 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human NK/T-cell lymphoma (PMID: 24225754) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR22403-57] (ab224703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR22403-57] (ab224703)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling NCR1 with ab224703 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in marginal zone of human tonsil (PMID: 24225754)  is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Western blot - Anti-NCR1 antibody [EPR22403-57] (ab224703)
    Western blot - Anti-NCR1 antibody [EPR22403-57] (ab224703)
    All lanes : Anti-NCR1 antibody [EPR22403-57] (ab224703) at 1/1000 dilution

    Lane 1 : NK-92 (human malignant non-hodgkin's lymphoma natural killer cell) whole cell lysate
    Lane 2 : Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa
    Observed band size: 35-46 kDa
    why is the actual band size different from the predicted?


    Exposure time: 70 seconds


    The expression profile observed is consistent with what has been described in the literature (PMID:9730896; 9314561).

     Negative control: Raji (PMID: 9730896).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-NCR1 antibody [EPR22403-57] (ab224703)
    Anti-NCR1 antibody [EPR22403-57] (ab224703)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-NCR1 antibody [EPR22403-57] (ab224703)

© 2021 Astana Biomed Group LLP. All rights reserved.