Anti-SIRT3 antibody (ab56214)
Key features and details
- Rabbit polyclonal to SIRT3
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-SIRT3 antibody
See all SIRT3 primary antibodies -
Description
Rabbit polyclonal to SIRT3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to C terminal residues of mouse SIRT3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified by antigen specific affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SIRT3 antibody (ab56214)Anti-SIRT3 antibody (ab56214) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 12 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The 48 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SIRT3.
