Gluconokinase Activity Assay Kit (Colorimetric) (ab211092)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 100 µU
Overview
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Product name
Gluconokinase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
Species reactivity
Reacts with: Mammals, Other speciesProduct overview
Gluconokinase Activity Assay Kit (Colorimetric) (ab211092) provides a simple, sensitive and convenient method for detecting gluconokinase activity in a variety of samples such as cell and tissue lysates, and even in prokaryotes such as E. coli. In this assay, Gluconokinase converts Gluconate into 6-Phosphate-D-Gluconate in an ATP dependent manner. 6-Phosphate-D-Gluconate and ADP in turn undergo a series of reactions to form an intermediate, which reacts with the probe to form a colored product with strong absorbance (OD 450 nm). The detection limit of this assay
Notes
Gluconokinase (GntK, ATP:D-gluconate 6-phosphotransferase, Gluconate Kinase; EC:2.7.1.12) is a key enzyme for degradation of gluconate. In E. coli and yeast, Gluconokinase phosphorylates gluconate into 6-Phosphate-D-Gluconate in an ATP dependent manner. Via the Hexose Monophosphate Shunt (HMS) pathway, 6- Phosphate-D-Gluconate generates ribose-5-phosphate, which is critical for nucleotides and nucleic acid synthesis.
Despite the widespread use of D-gluconate as acidity regulator in food and medicine, little is known of the mechanism of gluconate metabolism in humans.
Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests GntK Assay Buffer 1 x 25ml ATP (40 µmole) 1 vial GntK Converting Enzyme (10 U) 1 vial GntK Developer (10 U) 1 vial GntK Positive Control (200 mU) 1 vial GntK Probe (30 mg) 1 vial GntK Substrate (50 µmole) 1 vial NADH Standard (0.5 µmole) 1 vial -
Research areas
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Pathway
Carbohydrate acid metabolism; D-gluconate degradation. -
Sequence similarities
Belongs to the gluconokinase gntK/gntV family. - Information by UniProt
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Alternative names
- bA522I20.2
- C9orf103
- Chromosome 9 open reading frame 103
see all
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests GntK Assay Buffer 1 x 25ml ATP (40 µmole) 1 vial GntK Converting Enzyme (10 U) 1 vial GntK Developer (10 U) 1 vial GntK Positive Control (200 mU) 1 vial GntK Probe (30 mg) 1 vial GntK Substrate (50 µmole) 1 vial NADH Standard (0.5 µmole) 1 vial -
Research areas
-
Pathway
Carbohydrate acid metabolism; D-gluconate degradation. -
Sequence similarities
Belongs to the gluconokinase gntK/gntV family. - Information by UniProt
-
Alternative names
- bA522I20.2
- C9orf103
- Chromosome 9 open reading frame 103
see all
Images
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Gluconokinase Activity Assay Kit (Colorimetric) (ab211092)
Typical NADH standard calibration curve.
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Gluconokinase Activity Assay Kit (Colorimetric) (ab211092)Kinetic curves showing Gluconokinase activity detection in positive control (1 µL; included in kit), lysates from rat liver and kidkey (1 µg, respectively), lysates from MDA-MB-468 cells (1 µg) and E. coli (0.1 µg).
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Gluconokinase Activity Assay Kit (Colorimetric) (ab211092)Gluconokinase specific activity in lysates from rat liver and kidkey (1 µg, respectively), lysates from MDA-MB-468 cells (1 µg) and E. coli (0.1 µg).