Mouse IL-13R alpha 2 ELISA Kit (ab275894)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 26.31 pg/ml
- Range: 101.56 pg/ml - 6500 pg/ml
- Sample type: Cell culture media, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-13R alpha 2 ELISA Kit
See all IL-13 receptor alpha 2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6.4% Inter-assay Sample n Mean SD CV% Serum 3 7.1% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
26.31 pg/ml -
Range
101.56 pg/ml - 6500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94 83% - 103% Cell culture media 103 98% - 109% Hep Plasma 99 93% - 108% EDTA Plasma 106 102% - 111% Cit plasma 100 97% - 103% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL-13R alpha 2 ELISA kit (ab275894) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse IL-13R alpha 2 protein in mouse serum, plasma - citrate, plasma - heparin, plasma - edta, cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-13R alpha 2 with 26.31 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
IL-13R alpha 2 is a cytokine receptor involved in Th2 responses during parasitic infections and allergic asthma. IL-13R alpha 2 is released into bronchoalveolar lavage fluid from house dust mite allergen. It is also upregulated during heart failure, pulmonary hypertension, and in some forms of cancer.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IL-13R alpha 2 Capture Antibody 1 x 600µl 10X Mouse IL-13R alpha 2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse IL-13R alpha 2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4). -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily.
Contains 1 fibronectin type-III domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Cancer/testis antigen 19
- CD213a2
- CT19
see all -
Database links
- Entrez Gene: 16165 Mouse
- SwissProt: O88786 Mouse
- Unigene: 368330 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human IL-13R alpha 2 competitor curve comparison.Standard Curve comparison between Mouse IL-13R alpha 2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of mouse IL-13R alpha 2 standard curve in Sample Diluent NS.The IL-13R alpha 2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native L-13R alpha 2 in mouse serum and plasma samples.The concentrations of IL-13R alpha 2 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 20%, plasma (heparin) 10%, and plasma (EDTA) 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 29.01 ng/ml in serum, 12.39 ng/ml in plasma (citrate), 23.70 ng/ml in plasma (heparin), and 18.01 ng/ml plasma (EDTA).
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Interpolated concentrations of native IL-13R alpha 2 in mouse spleen tissue homogenate extract samples based on a 500 µg/mL extract load.The concentrations of IL-13R alpha 2 were measured in duplicate and interpolated from the IL-13R alpha 2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-13R alpha 2 concentration was determined to be 241.29 pg/mL in mouse spleen tissue homogenate extract.
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Sandwich ELISA - Mouse IL-13R alpha 2 ELISA Kit (ab275894)To learn more about the advantages of recombinant antibodies see here.
