Mouse/Rat Bcl-xL ELISA Kit (ab227899)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 28 pg/ml
- Range: 0.19 pg/ml - 12 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat
Overview
-
Product name
Mouse/Rat Bcl-xL ELISA Kit
See all Bcl-XL kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% L929 3 5.7% Inter-assay Sample n Mean SD CV% L929 5 9.1% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
28 pg/ml -
Range
0.19 pg/ml - 12 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 83 81% - 86% Mouse brain extract 105 94% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse, Rat -
Product overview
Mouse/Rat Bcl-xL ELISA Kit (ab227899) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Bcl-xL protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat Bcl-xL with 28 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse/Rat Bcl-xL Capture Antibody 1 x 600µl 10X Mouse/Rat Bcl-xL Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse/Rat Bcl-xL Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis. -
Tissue specificity
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death. -
Post-translational
modificationsProteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. -
Cellular localization
Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope. - Information by UniProt
-
Alternative names
- Apoptosis regulator Bcl X
- Apoptosis regulator Bcl-X
- Apoptosis regulator BclX
see all -
Database links
- Entrez Gene: 12048 Mouse
- Entrez Gene: 24888 Rat
- SwissProt: Q64373 Mouse
- SwissProt: P53563 Rat
- Unigene: 238213 Mouse
- Unigene: 10323 Rat
Images
-
Other - Mouse/Rat Bcl-xL ELISA Kit (ab227899)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of mouse/rat Bcl-xL standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native Bcl-xL in C2C12 cell extract, L929 cell extract based on a 300 µg/mL extract load, and PC-12 cell extract samples based on a 150 µg/mL extract loadThe concentrations of Bcl-xL were measured in duplicate and interpolated from the Bcl-xL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Bcl-xL concentration was determined to be 2.176 ng/mL in C2C12 cell extract, 6.010 ng/mL in L929 cell extract, and 6.022 ng/mL in PC-12 cell extract.
-
Interpolated concentrations of native Bcl-xL in mouse and rat brain tissue extract samples based on a 300 µg/mL extract loadThe concentrations of Bcl-xL were measured in duplicate and interpolated from the Bcl-xL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Bcl-xL concentration was determined to be 4.957 ng/mL in mouse brain tissue extract, and 3.067 ng/mL in rat brain tissue extract.
-
Sandwich ELISA - Mouse/Rat Bcl-xL ELISA Kit (ab227899)To learn more about the advantages of recombinant antibodies see here.
