Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TCP1 alpha/CCTA antibody [EPR4082] - BSA and Azide free (ab239946)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4082] to TCP1 alpha/CCTA - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TCP1 alpha/CCTA antibody [EPR4082] - BSA and Azide free
    See all TCP1 alpha/CCTA primary antibodies

  • Description

    Rabbit monoclonal [EPR4082] to TCP1 alpha/CCTA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab239946 is the carrier-free version of ab109126 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239946 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as TCP1 alpha

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-TCP1 alpha/CCTA antibody [EPR4082] - BSA and Azide free (ab239946)
    Immunoprecipitation - Anti-TCP1 alpha/CCTA antibody [EPR4082] - BSA and Azide free (ab239946)

    ab109126 at 1:50 dilution (5μg in 1mg lysate) immunoprecipitating TCP1 alpha/CCTA in ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate. 

     

    Lane 1: ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate

    Lane 2: PBS instead of ab109126 in ECV-304 whole cell lysate


    For western blotting, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as the secondary antibody at 1:1500 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109126).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 alpha/CCTA antibody [EPR4082] - BSA and Azide free (ab239946)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 alpha/CCTA antibody [EPR4082] - BSA and Azide free (ab239946)

    ab109126, at 1/100 dilution staining TCP1 in paraffin-embedded Human testis tissue, by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109126).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-TCP1 alpha/CCTA antibody [EPR4082] - BSA and Azide free (ab239946)
    Anti-TCP1 alpha/CCTA antibody [EPR4082] - BSA and Azide free (ab239946)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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