Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15827(B)] to DSTN - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free
    See all DSTN primary antibodies

  • Description

    Rabbit monoclonal [EPR15827(B)] to DSTN - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human bladder transitional cell carcinoma tissue.

  • General notes

    ab240301 is the carrier-free version of ab186754 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240301 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as Destrin.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)
    Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)

    ab186754 staining DSTN in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol.. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1:PBS only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186754).

  • Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)
    Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling DSTN with ab186754 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186754).

  • Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)
    Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF-7 cells labeling DSTN with ab186754 at 1/1000 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186754).

  • Flow Cytometry - Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)
    Flow Cytometry - Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)

    Flow cytometric analysis of 2% paraformaldehyde-fixed MCF-7 cells labeling DSTN with ab186754 at 1/360 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186754).

  • Immunoprecipitation - Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)
    Immunoprecipitation - Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)

    Western blot analysis of DSTN in A549 cell lysate immunoprecipitated with ab186754 at 1/50 dilution. 

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186754).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)

    Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling DSTN with ab186754 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186754).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)
    Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-DSTN antibody [EPR15827(B)] - BSA and Azide free (ab240301)

© 2021 Astana Biomed Group LLP. All rights reserved.